Wade Johnson

Assistant Professor of Chemistry

514 University Ave, Selinsgrove, PA 17870
Phone: (570) 372-4224
Fax: (570) 372-2751
E-mail: johnsonw@susqu.edu

B.S. in Chemistry from Hampden-Sydney College
M.S. in Inorganic Chemistry from Iowa State University
Ph.D. in Biochemistry from Iowa State University
Post-Doc in Biophysics at Pennsylvania State University

 

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Publications:
Review Articles:

Johnson, T. W. and J. H. Golbeck, Biological Incorporation of Foreign Quinones into the A1site of Photosystem I, review chapter for CRC Handbook of Organic Photochemistry and Photobiology, 2nd edition, Eds. W. M. Horspool and F. Lenci, 119, 2003.

Selected Research Articles:

Johnson, T. W. , L. Hui, F. Niels-Ulrik, D. A. Bryant, and J. H. Golbeck, Redox titration of the [2Fe-2S] clusters in the proteins CsmI, CsmJ, and CsmX in the chlorosome envelope of Chlorobium Tepidum, manuscript in preparation to be submitted to Biochemistry.

T. Wade Johnson, Eric Drago, Steven Romberger and Shelley Reppert, In Vivo Anthraquinone Incorporation into PS I complexes of Synechocystis sp. PCC 6803 Phylloquinone Lacking Mutants, manuscript in preparation to be submitted to Journal of biological chemistry.

W. Johnson, J. H. Golbeck, G. W. Brudvig and K. V. Lakshmi “The Arrangement of the Terminal [4Fe-4S] Clusters in Green Sulfur Bacteria: An EPR Study of Chlorobium Tepidum Reaction Centers”, (2007) Biophys. J. Manuscript in preparation.

XIVth International Congress on Photosynthesis July 22-27, 2007 in Glasgow Scotland, proceedings of the: Transient EPR Studies of In Vivo Uptake of Substituted Anthraquinones by Photosystem I in Phylloquinone Biosynthetic Pathway Mutants of Synechocystis sp. PCC 6803.
Sara Chirico, Eric Drago, John Golbeck, Wade Johnson and Art van der Est.

Priyangika K.M., W. Ruili, T. W. Johnson and G. Hastings, Time-resolved FTIR Difference Spectroscopy for the Study of Photosystem I Particles with Plastoquinone-9 Occupying the A1 Binding Site, Biochemistry, 45(42):12733-40, 2006

Johnson, T. W. and Nenad M. Kostic, Steric effects on the rate of hydrolysis by palladium(II) complexes of the C-terminal amide bond in a series of methionine-containing dipeptides AcMet-Aa, Journal of Serbian Chemistry Society, 69 (11), 887 – 899, 2004

Wang, R., V. Sivakumar, T. W. Johnson, and G. Hastings, FTIR Difference Spectroscopy in Combination with Isotope Labeling for Identification of the Carbonyl Modes of P700 and P700+ in Photosystem I, Biophysical Journal, 86, 1061-1073, 2004.

Johnson, T. W.* , S. Naithani, C. Stewartª, B. Zybailov, A. D. Jones, J. H. Golbeck, and P. R. Chitnis, Deletion of the menD and menE genes in the biosynthetic pathways in Synechocystis sp. PCC 6803, Biochimica et Biophysica Acta,, 1557, 67-67, 2003. *corresponding author; ªundergraduate researcher

Sergei, S., T. W. Johnson, P. Martinsson, P. R. Chitnis, and W. S. Struve, Ultrafast Primary Processes in Photosystem I Reaction Centers with a Foreign Quinone in the A1 Site, in preparation for submission to J. of Physical Chemistry B.

Zazubovich, V., S. Matsuzkia, T. W. Johnson, J. M. Hayes, P. R. Chitnis, and G. J. Small, Red antenna states of photosystem I from cyanobacterium Synechococcus elongatus: a spectral hole burning study, Chemical Physics, 275, 47-59, 2002

Johnson, T. W. , B. Zybailov, A. D. Jones, J. H. Golbeck, and P. R. Chitnis, Recruitment of a foreign quinone into the A 1 site of photosystem I. IV. In vivo replacement of the native quinone in the phylloquinone-less mutants of Synechocystis sp. PCC 6803 by externally supplied naphthoquinones, J. Biological Chemistry, 276, 39512-39521, 2001.

 Savikhin, S., P. Martinson, P. Chitnis, T. W. Johnson, W. Xu, and W. Struve, The kinetics of primary electron transfer steps in PS I reaction centers, Chemical Physics, 66, 662-664, 2001.

Johnson , T. W. , G. Shen, B. Zybailov, D. Kolling, R. Reategui, S. Beauparlant, I. R. Vassiliev, D. A. Bryant, A. D. Jones, J. H. Golbeck, and P. R. Chitnis, Recruitment of a foreign quinone into the A1 site of photosystem I. I genetic and physiological characterization of phylloquinone biosynthetic pathway mutants in Synechocystis sp. PCC 6803, J. Biological Chemistry, 275, 8523-8530, 2000.

Zybailov, B., A. van der Est ,, S. Zech, C. Teutloff , T. W. Johnson , G. Shen, R. Bittl, D. Stehlik, P. R. Chitnis, and J. H. Golbeck, Recruitment of a foreign quinone into the A1 site of photosystem I. II. Structural and functional characterization of phylloquinone biosynthetic pathway mutants by EPR and ENDOR, J. Biological Chemistry, 275, 8531-8539, 2000.

 Margus, R., T. W. Johnson, P. R. Chitnis, and G. Small, The red absorbing chlorophyll a antenna states of photosystem I: A hole burning study of Synechocystis sp. PCC 6803 and its mutants, J. Physical Chemistry, 104, 836-847, 2000.

Golbeck, J., B. Zybailov, I. Vassiliev, G. Shen, T.W. Johnson, R. Reageti, P., Chitnis, Deletion mutagenesis of essential genes in the phylloquinone biosynthetic pathway: Altered function of A(1) in the photosystem I, Photochemistry and Photobiology, 69, 71s, 1999

 Kaminskaia, N., T. W. Johnson, and N. M. Kostic, Hydrolysis of tryptophan-containing peptides by palladium (II) aqua complexes, J. of the American Chemical Society, 121 , 8663-8664, 1999.

 Reategui, R., T. W. Johnson , W. Xu, B. Zybailov, G. Shen, J. H. Golbeck, and P. R. Chitnis, Mutational analysis of photosystem I: function of phylloquinone, G. Garab (ed.), Photosynthesis: Mechanisms and Effects, Vol I, 515-520, 1998, Kluwer Academic Publishers, Netherlands.

Peterson, P. E., M. Abu-Omar, T. W. Johnson, R. Parham, D. Goldin, C. Henry II, A. Cook, and K. M. Dunn, Ab Initio predictions of vibrational frequencies for cationic species, J. Physical Chemistry, 99, 5927-5933, 1995.


Selected Presentations:

Johnsonw, T. W., Steven Romberger ª , Erica L. Wagnerª , John Golbeck, and Art Van der Est, “ Synechocystis sp. PCC 6803 Mutants Lacking Phylloquinone in PSI Utilize Alternative Anthraquinone SupplementsPoster, Eastern Regional Photosynthesis Meeting, Woods Hole, Massachusetts April, 2005. ªundergraduate researcher

Velautham Sivakumar, Ruili Wang, T. Wade Johnson and Gary Hastings, “ A1 Reduction in Intact Cyanobacterial Photosystem I Studied Using Time-resolved Step-scan Fourier Transform Infra-red Difference Spectroscopy In Combination With Site Directed Mutagenesis and Quinone Exchange Experiments”. Abstract and poster presented at the World Photosynthetic Conference, Montréal, Quebec, 2004

Johnson, T. W. , L. Hui, F. Niels-Ulrik, D. A. Bryant, and J. H. Golbeck, Redox titration of the [2Fe-2S] clusters in the proteins CsmI, CsmJ, and CsmX in the chlorosome envelope of Chlorobium Tepidum”, poster , Photosynthesis Gordon Conference, Newport, Rhode Island, June, 2003.

Johnson, T. W. , M. Harris ª , S. McVeyª , N. U. Frigaard, H. Li, D. Bryant, J. H. Golbeck “Time Resolved and CW X-band EPR Characterization of Chlorobium tepidum Wild Type and Mutants in Whole Cells, Membranes and Chlorosomes“, poster, Photosynthesis Gordon Conference, Newport, Rhode Island, June, 2002. ªundergraduate researcher

Johnson, T. W. , R. Cohen, W. Xu, P. R. Chitnis, J. Golbeck “A 0 mutants in Photosystem I” oral presenter, Eastern Regional Photosynthesis Meeting, Woods Hole, Massachusetts April, 2002.

Johnson , T. W. , Nathinia, S. B. Zybailov, I. R. Vassiliev, J. H. Golbeck, and P. R. Chitnis “Recruitment of a Foreign Quinone into the A 1 Site of Photosystem I: The Biosynthetic Pathway of Phylloquinone and In vivo Replacement of the Native Quinone in Synechocystis sp. PCC 6803,” invited speaker, Hampden-Sydney College, Hampden-Sydney, Virginia, October, 2001.

Johnson , T. W. , B. Zybailov, I. R. Vassiliev, A. D. Jones, J. H. Golbeck, and P. R. Chitnis “Recruitment of a Foreign Quinone into the A 1 Site of Photosystem I: a) Inactivation of the menD and menE Genes in the Biosynthetic Pathway of Phylloquinone b) In vivo Replacement of the Native Quinone in the Phylloquinone-less Mutants of Synechocystis sp. PCC 6803 by Externally Supplied Naphthoquinone,” poster, Photosynthesis Gordon Conference, Meriden, New Hampshire, June, 2000.

Johnson , T. W. , G. Shen, B. Zybailov, R. Reategui, I. R. Vassiliev, D. A. Bryant, A. D. Jones, J. H. Golbeck, and P. R. Chitnis “ Recruitment of a Foreign Quinone into the A 1 Site of Photosystem I: I Genetic and Physiological Characterization of Phylloquinone Biosynthetic Pathway Mutants in Synechocystis sp. PCC 6803,” poster, Photosynthesis Gordon Conference, Henniker, New Hampshire, June, 1999.

Johnson , T. W. , B. Zybailov, R. Reategui, I. R. Vassiliev, A. D. Jones, J. H. Golbeck, and P. R. Chitnis “Genetic and Physiological Characterization of Phylloquinone Biosynthetic Pathway Mutants in Synechocystis sp. PCC 6803,” poster and oral presenter, Mid-West Photosynthetic meeting, Turkey Run Indiana, October, 1999.

Johnson, T. W. and N. M. Kostic “C-Terminal Amide Bond in a Series of Dipeptides Acmet-AA,” poster, 212 th National ACS Meeting, Orlando, Florida, August, 1996.

Johnson, T. W. and N. M. Kostic, “Selective Hydrolysis of Methionine Containing Peptides;” poster, Midwest Bioinorganic Chemistry Workshop, Minneapolis, Minnesota, June, 1995.

 

 

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